Modified Oligos

Synbio Technologies provides oligo synthesis and modifications. Our specialized services include oligonucleotides modified with special bases, dU/dI, phosphorothioates, phosphorylation, spacers, amino linkers, disulfide linkers, biotin, etc. Synbio Technologies also provides multiple fluorescent groups and quenchers at 3’, 5’ or designated sites specified by the customer. This includes FAM, HEX, ROX, BHQ, Texas Red, and much more. The resulting oligos can be used in a variety of applications, including PCR, cloning, sequence verification, and gene detection.

Competitive Advantages

  • Comprehensive Modifications and Labeling: Large-scale sequencing verifying the average mutation rate to be lower than 1/1000
  • Strict Quality Control: Stringent ISO9001 & ISO13485 Certified Processing for oligo synthesis, meticulously monitored and advanced traceability
  • Multiple Purification Options: Include desalt, PAGE Plus, PAGE, RP-HPLC, IE-HPLC and Dual HPLC
  • Technical Support: Professional teams experienced in oligo synthesis and various modifications offer support throughout the production progress

Services Specifications

General Modifications

Single Labeling

Dual/Multiple Labeling

General Modifications

Synbio Technologies provides a series of general modifications with the high purity and competitive prices. We accomplish this by focusing on the requests of modification with special chemical classifications. Browse general modifications listed below:

Classification Modifications Purification
Modified Bases
dU, dI, 5-Methyl dC
HPLC/PAGE
DBCO
5’ DBCO, 3’ DBCO, dT-DBCO
HPLC/PAGE
AMCA
5’ AMCA, 3’ AMCA, dT-AMCA
HPLC/PAGE
Phosphorothioates
SPO3
HPLC/PAGE
Amino Linkers
5’Amino C6, dT-Amino C6, 3’Amino C7, 5’Amino C12
HPLC/PAGE
Biotin
5’Biotin, 3’Biotin, dT-Biotin
HPLC/PAGE
Digoxigenin
5’Digoxigenin, 3’Digoxigenin, dT-Digoxigenin
HPLC/PAGE
Phosphorylation
5’ PO4, 3’ PO4
HPLC/PAGE
Hydrosulphonyl
5’ HS-C6, 3’ HS-C3
HPLC/PAGE
Locked Nuleic Acid
LNA (A/G/C/U)
HPLC/PAGE
Fluoro Bases
2’-F-2’-dA, 2’-F-2’-dC, 2’-F-2’-dG, 2’-F-2’-dU
HPLC/PAGE
Spacer
dSpacer, Spacer C3, Spacer C9, Spacer C18
HPLC/PAGE

Standard Deliverables

  1. Lyophilized dry DNA oligo powder
  2. 1 tube (Transparent or dark) or 96-well and 384-well plate
  3. Certificate of Analysis (COA) including sequencing information, OD, Tm, etc.

Single Labeling

Synbio Technologies provides multiple fluorescent groups at 3’, 5’ or designated sites to comply with our customers’ requests. This includes FAM, HEX, ROX, BHQ and Texas Red, etc.

Dye/Quenchers 3′- 5′- Designated Sites Purification
FAM
HPLC/PAGE
TET
HPLC/PAGE
JOE
HPLC/PAGE
HEX
HPLC/PAGE
SIMA
HPLC/PAGE
TAMRA
HPLC/PAGE
ROX
HPLC/PAGE
Cy5
HPLC/PAGE
Cy3
HPLC/PAGE
Quasar670
HPLC/PAGE
Quasar570
HPLC/PAGE
DyLight547
HPLC/PAGE
DyLight647
HPLC/PAGE
Texas Red
HPLC/PAGE
BHQ-1
HPLC/PAGE
BHQ-2
HPLC/PAGE
BHQ-3
HPLC/PAGE
Eclipse
HPLC/PAGE
DABCYL
HPLC/PAGE

Standard Deliverables

  1. Lyophilized dry DNA oligo powder
  2. 1 tube (Transparent or dark) or 96-well and 384-well plate
  3. Certificate of Analysis (COA) including sequencing information, OD, Tm, etc.

Dual/Multiple Labeling

Despite of single fluorescent group, Synbio Technologies also offers dual/multiple labeling services including TAMRA, BHQ, DABYCL, ELCISPE, etc. The most common Dyes and Quenchers are listed below:

Dyes Quenchers Purification
5’6-FAM
3’TAMRA, 3’BHQ-1, 3’Eclipse
HPLC/PAGE
5’TET
3’TAMRA, 3’BHQ-2, 3’DABCYL
HPLC/PAGE
5’HEX
3’TAMRA, 3’BHQ-1, 3’BHQ-2, 3’DABCYL
HPLC/PAGE
5’TAMRA
3’Eclipse, 3’BHQ-1, 3’BHQ-2, 3’DABCYL
HPLC/PAGE
5’ROX
3’Eclipse, 3’BHQ-1, 3’BHQ-2, 3’DABCYL
HPLC/PAGE
5’CY5
3’BHQ-1, 3’BHQ-2, 3’BHQ-3, 3’DABCYL
HPLC/PAGE
5’CY3
3’BHQ-2
HPLC/PAGE
5’JOE
3’Eclipse, 3’BHQ-1, 3’BHQ-2. 3’DABCYL, 3’TAMRA
HPLC/PAGE
5’JOE
3’BHQ-2
HPLC/PAGE

Standard Deliverables

  1. Lyophilized dry DNA oligo powder
  2. 1 tube (Transparent or dark) or 96-well and 384-well plate
  3. Certificate of Analysis (COA) including sequencing information, OD, Tm, etc.
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