DAPGreen - Autophagy Detection

Description

DAPGreen is used to detect autophagy in live cells. Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. DAPGreen, is a small fluorescent molecule, detects autophagosomes and autolysosomes possibly by a mechanism that the dye is incorporated into autophagosome during double membrane formation due to structure features, and then emits fluorescence under hydrophobic conditions. DALGreen is cell permeable, has no requirement of transfection method, and enables live cell imaging with fluorescence microscopy and quantitative assay by flow cytometry. For monitoring autolysosome, DALGreen [D675] is recommend since it allows detection of phagosome-lysosome fusion.