Glutamine Assay Kit-WST

Description

Glutamine, a source of ?-ketoglutarate (one of the tricarboxylic acid (TCA) cycle intermediates), is an important substance used for energy production and the syntheses of nucleic acids and other amino acids. Glutaminolysis, the process by which glutamine as a substrate is converted to ?-ketoglutarate, is upregulated in cancer cells. A study reports that glutaminolysis has largely contributed to scavenging of reactive oxygen species and reduction of oxidized glutathione.

The Glutamine Assay Kit-WST can quantify glutamine, a substrate of energy metabolism. This kit allows you to quantify glutamine present in culture medium or intracellular glutamine by WST reduction reaction. The lowest concentration of glutamine which can be quantified is 5 ?mol/l. This kit can be used with 96-well microplates, which makes it possible to analyze multiple specimens.