NADP/NADPH Assay Kit

Description

Nicotinamide adenine dinucleotide phosphate (NADP) is an important cofactor involved in reactions in the pentose phosphate pathway, one of the metabolic pathways in cells. NADP exists as an oxidized form NADP+ and a reduced form NADPH in cells. NADPH is important not only for biosynthesis of fatty acids and cholesterol but also for generation of reduced glutathione (GSH). In addition, a recent study suggests that NADP+/NADPH is associated with the extension of life span by carbohydrate restriction.

NADP/NADPH Assay Kit-WST enables quantitation of the amount of total NADP+/NADPH, NADPH and NADP+ in cells and measurement of their ratio. The intracellular NADPH levels can be quantitated selectively by heat treatment of cell lysate with the extraction buffer in this kit. The intracellular NADP+ levels can also be determined by subtracting the amount of NADPH assayed from the amount of total NADP+/NADPH.