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Premix Ex Taq (Probe qPCR)

Premix Ex Taq (Probe qPCR) is a 2X premix designed for probe-based real-time PCR (qPCR), including 5' nuclease assays. It combines TaKaRa Ex Taq HS, a hot-start DNA polymerase controlled by an anti-Taq antibody, with a buffer optimized for real-time PCR. The hot-start design helps prevent non-specific amplification caused by mispriming during reaction setup, supporting accurate quantification and detection over a broad dynamic range. The premix also contains dNTPs, Mg2+ and Tli RNase H. Primers and probe are not included.
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