Elastase, =50 U/mg
Key Features and Details
SYNONYMS: Elastase from hog pancreas; Pancreatopeptidase E
CAS: # 2594837
EC No: 254-453-6
MDL No: MFCD00130998
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Elastase Application Notes Elastase was used to study human emphysema by induction of emphysema in rodents. It has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Investigate the purification and partial characterization of the pancreatic proteolytic enzymes
Base Catalog Number: 100617
Alternate Names: Elastase from hog pancreas; Pancreatopeptidase E
Biochemical Physiological Actions : Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
CAS : # 2594837
EC No : 254-453-6
Format : Solution
Formulation Details : Aqueous suspension with 0.01% sodium azide as preservative
Grade : 2× Crystallized
Hazard Statements : H315-H319-H335
MDL No : MFCD00130998
PPE : Dust mask, Eyeshields, Faceshields, Gloves
Preparation Method : Elastase is prepared from porcine pancreas. It is lyophilized and water soluble. Elastase is chromatographically prepared by the method of Narayanan and Anwar. In the method, 2 times crystalline elastase is adsorbed on a column of DEAE-Sephadex A50 to separate elastase and non-specific protein components. The elastase component is further purified by chromatography on a column of CM-Cellulose using a sodium chloride gradient to elute the elastase. The latter is dialyzed until chloride-free and then lyophilized. During its preparation the elastase is held below a pH of 5.5 for greater than 24 hours. Two times crystallized from the euglobin fraction of porcine pancreas by the method of Lewis et al. Does not contain trypsin or chymotrypsin.
RTECS No : JX7069000
Safety Symbol : GHS07
Source : Porcine pancreas
Specific Activity : Activity =50 u/mg protein
Unit Definition : One unit will solublize 1 mg of elastin in 20 minutes at 37 °C, pH 8.8.