Ribonuclease A (bovine)
Key Features and Details
SYNONYMS: Pancreatic Ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3' pyrimidinooligonucleotidohydrolase
CAS: # 9001-99-4
MOLECULAR WEIGHT: 13700 (based on Amino acid sequence)
EC No: 232-646-6
MDL No: MFCD00132181
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Base Catalog Number: 101076
Alternate Names: Pancreatic Ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3' pyrimidinooligonucleotidohydrolase
Biochemical Physiological Actions : Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3 phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2'-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.
CAS : # 9001-99-4
EC No : 232-646-6
Extinction Coefficient : 7.3 (280 nm) (Lit.)
Isoelectric Point : pH 9.45 (Lit.)
MDL No : MFCD00132181
Molecular Weight : 13700 (based on Amino acid sequence)
pH : Optimum pH: 7.0 - 7.5
PPE : Eyeshields, Gloves, Repirator Filter
RTECS No : RF0760000
Solubility : Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Source : Bovine Pancreas
Specific Activity : =50 Kunitz u/mg
Specificity : The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
Unit Definition : That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity at 25 °C and pH 5.0.