Ribonuclease, beef pancreas, 100 mg
Key Features and Details
SYNONYMS: RNase A; E.C. 22.214.171.124; Ribonuclease I; Ribonucleate 3'-pyrimidinooligonucleotidohydrolase; Pancreatic Ribonuclease; Endoribonuclease I
CAS: # 9001-99-4
MOLECULAR WEIGHT: ~13,700 (Lit.)
EC No: 232-646-6
MDL No: MFCD00132181
RNase A is an endoribonuclease that attacks at the 3' phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. Application Notes Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the tissue-specific expression of ruminant pancreatic type ribonucleases. Ribonuclease A from bovine pancreas has also been used in a study to investigate pancreas-specific glycosylated protein disulphide-isomerase binding to misfolded proteins and peptides. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Purification of DNA | RNA sequence analysis | RNase protection assays | Remove unspecifically bound RNA | Hydrolyze RNA contained in protein samples
Base Catalog Number: 101075.1
Alternate Names: 101075.1
Biochemical Physiological Actions : Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides.
CAS : # 9001-99-4
EC No : 232-646-6
Extinction Coefficient : E1%280 = 7.3(Lit.)
Hazard Statements : H317
MDL No : MFCD00132181
Molecular Weight : ~13,700 (Lit.)
pH : Optimum pH: 7.0 - 7.5(Lit.)
PPE : Eyeshields, Gloves, respirator filter
RTECS No : RF0760000
Solubility : Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Specific Activity : ~70 Kunitz u/mg solid
Unit Definition : One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C and pH 5.0.