Albumin bovine, fraction V,=95%, 25 g
Key Features and Details
SYNONYMS: Bovine Serum Albumin; Bovine Plasma Albumin; BSA
CAS: # 9048-46-8
MOLECULAR WEIGHT: The has been published at 66,338. Other reported weights range from 66,120 to 66,430.
EC No: 232-936-2
MDL No: MFCD00130384
Albumins are a group of acidic proteins which occur in the body fluids and tissues of mammals and in some plant seeds. Serum and plasma albumin is carbohydrate free and comprises 55-62% of the protein present. However, only about 40% of the total albumin in the body is in the circulating plasma at one time with the remainder being in extracellular spaces with which there is, in general, equilibration about every 24 hours. Bovine albumin is a single polypeptide chain consisting of approximately 583 to 595 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group. Application Notes Albumin binds water, Ca2+, Na+ and K+. Due to a hydrophobic cleft, albumin binds fatty acids, bilirubin, hormones and drugs. The main biological function of albumin is to regulate the colloidal osmotic pressure of blood and to a lesser degree to provide cellular nutrition. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Albumin is used to solubilize lipids, stabilize protein solutions, and is also used as a blocking agent in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present such as electrophoresis. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Regulate the colloidal osmotic pressure of blood used to solubilize lipids | Stabilize protein solutions | Blocking agent
Base Catalog Number: 10370325
Alternate Names: Bovine Serum Albumin; Bovine Plasma Albumin; BSA
Biochemical Physiological Actions : Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
CAS : # 9048-46-8
EC No : 232-936-2
Hazard Statements : H317
MDL No : MFCD00130384
Molecular Weight : The has been published at 66,338. Other reported weights range from 66,120 to 66,430.
Optical Rotation: [a]259: -61°; [a]264: -63°(lit.)
pH : (1% soln in 0.15 M NaCl) 5.0 - 5.4
PPE : Eyeshields, Gloves, respirator filter
Preparation Method : The manufacturing process is performed under non-denaturing conditions so as to yield a product with a native fatty acid profile. Exogenous short chain fatty acid stabilizers are never added. In procedures which require the True Cohn Fraction V Powder, this is the only grade to use.
Purity : =95%
RTECS No : MT6446000
Solubility : Soluble in water.