TMB is a chromogenic substrate for Horse Radish Peroxidase (HRP). MP Biolomedicals TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver reproducible results
Metabolism of the TMB substrate by HRP creates a soluble bluish reaction product that may be quantified by measuring absorbance at 370 or 665 nm. Reaction can be stopped by addition of 1 volume of 1N hydrocloric or sulfurc acid to yield a yellow reaction product that can be measured by absorbance at 450 nm (within one hour).
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