2-Iminothiolane Hydrochloride
Application Notes
2-Iminiothiolane hydrochloride is a thiolating reagent that is used in the preparation of disulfide and thioether linked conjugates, such as for protein crosslinking. It has a preference for primary amino groups and reacts at pH 7-10 to give amidine compounds which contain free sulhydryl groups. The amidine linkage preserves the original primary amine positive charge. A mechanistic study of the reaction of 2-iminothiolane with amino groups in peptides and proteins has been published. 2-Iminothiolane has also been utilized to prepare glycoprotein-antibody conjugates, streptavidin-antibody conjugates, and avidin-protein nanoparticle conjugates.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Protein modification and cross-linking reagent