3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide, cell culture reagent

Key Features and Details

3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide

SYNONYMS: MTT; Thiazolyl Blue;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;Methylthiazolyldiphenyl-tetrazolium bromide
CAS: # 298-93-1
MOLECULAR WEIGHT: 414.325 g/mol
EC No: 206-069-5
MDL No: MFCD00011964
SKU 02194592-CF Categories , , , Tag

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3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide Application Notes 3-(4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Colorimetric assay; Cell viability; cell culture
SKU: 02194592-CF
Base Catalog Number: 194592
Alternate Names: MTT; Thiazolyl Blue;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;Methylthiazolyldiphenyl-tetrazolium bromide
Beilstein Registration No : 4081397
CAS : # 298-93-1
EC No : 206-069-5
Grade : Cell Culture
Hazard Statements : H315-H319-H335-H341
MDL No : MFCD00011964
Melting Point : 195 deg C (decomposes)
Molecular Formula : C18H16BrN5S
Molecular Weight : 414.325 g/mol
PPE : Eyeshields, Gloves, respirator filter
Purity : ~98%
RTECS No : XF8060000
Safety Symbol : GHS07, GHS08
Solubility : Solubility in 2-methoxyethanol, 20 mg/mL; ethanol, 20 mg/mL
UV Visible Absorbance : 376 ± 2 nm; 242 ± 2 nm ?max (methanol)
Vapor Pressure : 5.11X10-11 mm Hg at 25 deg C /Estimated/

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