3,3',5,5'-Tetramethylbenzidine
Key Features and Details
3,3′,5,5′-Tetramethylbenzidine
SYNONYMS: TMB; TMB substrate; BM blue; Sure Blue TMB; TMB Blotting Plus
CAS: #
54827-17-7
MOLECULAR FORMULA: C16H20N2
MOLECULAR WEIGHT: 240.35 g/mol
BEILSTEIN REGISTRY No.: 2808541
EC No: 259-364-6
MDL No: MFCD00007748
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PRODUCT DESCRIPTION
3,3',5,5'-Tetramethylbenzidine
Application Notes
3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases. It is useful in gel staining procedure for low levels of home-associated peroxidases and for enzyme immunoassay of horseradish peroxidase.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Peroxidase substrate suitable for use in ELISA
SPECIFICATIONS
SKU: 02190277-CF
Base Catalog Number: 190277
Alternate Names: TMB; TMB substrate; BM blue; Sure Blue TMB; TMB Blotting Plus
Beilstein Registration No
: 2808541
CAS
: #
54827-17-7
EC No
: 259-364-6
Format
: Powder
Hazard Statements
: H315-H319-H335
MDL No
: MFCD00007748
Melting Point
: 334 to 336° F (NTP, 1992)
Molecular Formula
: C16H20N2
Molecular Weight
: 240.35 g/mol
PPE
: Dust mask, Eyeshields, Faceshields, Gloves
Purity
: =99%
RTECS No
: DV2300000
Safety Symbol
: GHS07
Solubility
: less than 1 mg/mL at 68° F (NTP, 1992)
Typical Working Concentration
: Solutions for HRP substrate: Dissolve 1.5 mg TMB in 0.1 mL of DMSO. Then add 9.9 ml of 0.1 M sodium acetate (pH 6.0). Finally, add hydrogen peroxide to a concentration of 0.01% and filter through a Whatman #1 paper filter. Prepare solutions fresh daily. Discard any remaining solution at the end of the day.
