Key Features and Details
SYNONYMS: Apase ; Orthophosphoric-monoester phosphohydrolase (acid optimum); Phosphatase, Acid from wheat germ
CAS: # 9001-77-8
MOLECULAR WEIGHT: 114.144 g/mol
EC No: 232-630-9
MDL No: MFCD00131847
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Acid Phosphatase Application Notes Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Acid phosphatase from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid
Base Catalog Number: 153876
Alternate Names: Apase ; Orthophosphoric-monoester phosphohydrolase (acid optimum); Phosphatase, Acid from wheat germ
Biochemical Physiological Actions : Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.07.0 and the optimal temperature is 45 °C.
CAS : # 9001-77-8
EC No : 232-630-9
Format : Lyophilized powder
MDL No : MFCD00131847
Molecular Weight : 114.144 g/mol
PPE : Eyeshields, Gloves, respirator filter
Source : Wheat Germ
Unit Definition : One unit will hydrolyze 1.0 µmole of o-carboxyphenyl phosphate per minute at 25 °C, pH 5.0.