Key Features and Details
SYNONYMS: Alcohol oxidase: oxygen oxido-reductase; E.C. 220.127.116.11
CAS: # 9073-63-6
MOLECULAR WEIGHT: 191.315 g/mol
EC No: 231-791-2
MDL No: MFCD00130452
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Alcohol Oxidase Application Notes Alcohol oxidase is used for qualitative and quantitative determination of methanol or ethanol (blood alcohol) by colorimetric procedures. Unlike present ADH based assays for ethanol in biological specimens, no labile cofactors or trapping agents are required and considerably less enzyme is used per assay. Quantitative determination of alcohol using an oxygen electrode (enzyme electrode) is also possible. With or without catalase, alcohol oxidase can be used to remove alcohol or scavenge oxygen. Using dilute methanol, sensitive samples can be sterilized through release of the products formaldehyde and hydrogen peroxide. The enzyme can also be used to generate H2O2 in radio-iodine labelling procedures. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Colorimetric determination of blood alcohol
Base Catalog Number: 190155
Alternate Names: Alcohol oxidase: oxygen oxido-reductase; E.C. 18.104.22.168
Biochemical Physiological Actions : Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well.
CAS : # 9073-63-6
EC No : 231-791-2
Format : Frozen Liquid
Formulation Details : Supplied in 30% Sucrose; 0.1 M Potassium Phosphate Buffer at pH 8.0.
MDL No : MFCD00130452
Molecular Weight : 191.315 g/mol
PPE : Eyeshields, Gloves, Respirator filter
Source : Pichea Pastoris
Specific Activity : =10 u/mg Protein
Specificity : The enzyme is specific for short-chain; linear aliphatic alcohols and oxidizes the following substrates with a relative velocity rate: methanol = ethanol > n-propanol > n-butanol. It also catalyzes the oxidation of allyl and propargyl alcohol; methyl and ethyl mercaptan and fromaldehyde at slower rates. Branched chain alcohols; C2-aldehydes or higher; ketones and organic acids are not substrates. However; some of these reagents can give false positive responses due to trace contamination with alcohol. Oxygen is the only known hydrogen acceptor. At air saturation; the apparent KM for alcohol oxidase is 0.7 mM using methanol and 9 mM using ethanol as the substrate. In oxygen saturated solutions the KM values are higher. The turnover number is 20;000 sec-1.
Unit Definition : One unit will oxidize 1.0 µmole of methanol to formaldehyde per minute at pH 7.5 at 25 °C.