Alkaline phosphatase

Key Features and Details

Alkaline Phosphatase

SYNONYMS: EC 3.1.3.1; Alkaline phosphomonoesterase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); CIAP
CAS: # 9001-78-9
MOLECULAR WEIGHT: 496.569 g/mol
EC No: 232-631-4
MDL No: MFCD00131849

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PRODUCT DESCRIPTION

Alkaline Phosphatase Application Notes Alkaline phosphatase is used to remove 5'-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations. It is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA's, and other antibody based detection assays. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Used to remove 5'-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations | Used in immunoblotting; ELISA's; and other antibody based detection assays
SPECIFICATIONS
SKU: 02190680-CF
Base Catalog Number: 190680
Alternate Names: EC 3.1.3.1; Alkaline phosphomonoesterase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); CIAP
Biochemical Physiological Actions : Catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5'-OH can be phosphorylated with polynucleotide kinase and ?32P-ATP to produce ?32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.
CAS : # 9001-78-9
EC No : 232-631-4
Format : Powder
Isoelectric Point : 5.7(Lit.)
MDL No : MFCD00131849
Molecular Weight : 496.569 g/mol
PPE : Eyeshields, Gloves, respirator filter
Source : Calf Intestine
Specific Activity : =1.0 u/mg
Unit Definition : That amount of enzyme causing the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 25 °C and pH 9.6.

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