Alkaline phosphatase
Key Features and Details
From E. coli
SYNONYMS: Orthophosphoric Monoester Phosphohydrolase
CAS: #
9001-78-9
MOLECULAR WEIGHT: 496.569 g/mol
EC No: 232-631-4
MDL No: MFCD00131849
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PRODUCT DESCRIPTION
Alkaline Phosphatase
Application Notes
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Conjugation | Protein Labelling
SPECIFICATIONS
SKU: 02199817-CF
Base Catalog Number: 199817
Alternate Names: Orthophosphoric Monoester Phosphohydrolase
Biochemical Physiological Actions
: Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
CAS
: #
9001-78-9
EC No
: 232-631-4
Format
: Ammonium Sulfate Suspension
MDL No
: MFCD00131849
Molecular Weight
: 496.569 g/mol
PPE
: Eyeshields, Gloves, respirator filter
Source
: E. Coli
Specific Activity
: =30 u/mg protein , Activity- ~1000 u/mL
Unit Definition
: One unit will hydrolyze 1 µmole of 4-nitrophenyl phosphate per minute at pH 10.4, 37 °C.
