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Carbonic anhydrase
Key Features and Details
Carbonic Anhydrase
SYNONYMS: Carbonate Dehydratase; Carbonate Hydrolyase
CAS: #
9001-03-0
MOLECULAR WEIGHT: 397.751 g/mol
EC No: 232-576-6
MDL No: MFCD00081467
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PRODUCT DESCRIPTION
Carbonic Anhydrase
Application Notes
Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like a-crystallin and prevent aggregation. The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Enzymes | Inhibitors | Substrates
SPECIFICATIONS
SKU: 02153879-CF
Base Catalog Number: 153879
Alternate Names: Carbonate Dehydratase; Carbonate Hydrolyase
Biochemical Physiological Actions
: Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications for various pathologies such as glaucoma, epilepsy and Parkinsons disease.
CAS
: #
9001-03-0
EC No
: 232-576-6
Format
: Powder
Hazard Statements
: H317
MDL No
: MFCD00081467
Molecular Weight
: 397.751 g/mol
PPE
: Eyeshields, Gloves, Respirator filter
Source
: Bovine Erythrocytes
Specific Activity
: >2000 u/mg solid
Unit Definition
: The Unit is determined by the electrometic method in which the time required (in seconds) for a saturated CO2 solution to lower the pH of 0.02M Tris-HCI buffer from 8.3 to 6.3 at 0 °C is determined. The time without enzyme is recorded at To; with enzyme T. One unit equals 2X (To-T)/T using the electrometric assay.
