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CHAPS
Key Features and Details
Nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions
For protein solubilization in non denaturing isoelectric focusing and 2-D electrophoresis
SYNONYMS: 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate; N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl] amino]-1-propanaminium inner salt
CAS: #
75621-03-3
MOLECULAR FORMULA: C32H58N2O7S
MOLECULAR WEIGHT: 614.9
MDL No: MFCD00012116
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PRODUCT DESCRIPTION
CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents.
Application Notes
CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Detergent for solubilizing membrane proteins
SPECIFICATIONS
SKU: 02190319-CF
Base Catalog Number: 190319
Alternate Names: 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate; N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl] amino]-1-propanaminium inner salt
Aggregation Number
: 4-14 (0-0.1 M Na+)
CAS
: #
75621-03-3
Critical Micelle Concentration
: 6-10 mM
Hazard Statements
: H315-H319-H335-H360D
MDL No
: MFCD00012116
Melting Point
: 265°C (Lit.)
Molecular Formula
: C32H58N2O7S
Molecular Weight
: 614.9
pH
: 4.0 - 7.0 (10% aq soln)
Purity
: =98%
Safety Symbol
: GHS07, GHS08
Solubility
: Soluble in water (100 mg/mL - clear, colorless to slightly yellow solution).
Typical Working Concentration
: Concentrations between 1-4% (v/v) are typically used in an isoelectric focusing gel.
