Collagen, Type I from calf skin

Key Features and Details

Collagen

SYNONYMS: Collagensoluble; Collagen type I
CAS: # 9007-34-5
EC No: 232-697-4
MDL No: MFCD00130825

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PRODUCT DESCRIPTION

Collagen Application Notes Collagen Type I has been used as a scaffold for the growth of stem cells in vitro, in a wide variety of biomaterial engineering studies. Collagen type I may be used in research of Idiopathic pulmonary fibrosis (IPF). Robust expression of collagen-type 1 is one distinctive feature of IPF. Additionally, collagen-type I has been used in studies on the effect of endoplasmic reticulum (ER) stress from IPF on myofibroblastic differentiation of lung fibroblasts. Collagen-type 1 soluble in acidic solution produces three dimensional scaffolding useful in bioengineering and cell culture applications where biomaterials are needed to replace native collagen extracellular matrices. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Bioengineering
SPECIFICATIONS
SKU: 02160084-CF
Base Catalog Number: 160084
Alternate Names: Collagensoluble; Collagen type I
CAS : # 9007-34-5
EC No : 232-697-4
Format : lyophilized, Salt-free, Soluble
MDL No : MFCD00130825
PPE : Eyeshields, Gloves, respirator filter
Preparation Method : It is prepared according to the method of Gallop and Seifter. In the method, fresh calf skin is extracted with 0.5 M NaOAc to remove non-collagen proteins. The soluble collagen is extracted with 0.075 M Sodium citrate, pH 3.7, and precipitated as fibrils by dialysis against 0.02 M Na2HPO4.It is prepared according to the method of Gallop and Seifter. In the method, fresh calf skin is extracted with 0.5 M NaOAc to remove non-collagen proteins. The soluble collagen is extracted with 0.075 M Sodium citrate, pH 3.7, and precipitated as fibrils by dialysis against 0.02 M Na2HPO4.
Source : Calf skin
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