Collagenase type 3, >100 U/mg
Key Features and Details
Collagenase Type 3
SYNONYMS: Clostridiopeptidase A; EC 188.8.131.52
CAS: # 9001-12-1
EC No: 232-582-9
MDL No: MFCD00130830
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Collagenase Type 3 Application Notes Collagenase has found widespread application in the isolation of specific cell types from attendant connective tissue. Most preparations contain collagenase, non-specifc protease, and clostripain activities. These enzymes act synergistically to degrade collagen and other intercellular material. Crude Collagenases are suitable for isolation of cells from many types of animal tissue. The wide spectrum of activity of crude collagenase makes it especially useful as a debriding agent. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Cell Dissociation | Cell Lysis
Base Catalog Number: 150704
Alternate Names: Clostridiopeptidase A; EC 184.108.40.206
Biochemical Physiological Actions : Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N',N'-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Crude collagenases are mixtures of enzymes (mostly proteases) secreted by C. histolyticum. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa.
CAS : # 9001-12-1
EC No : 232-582-9
Format : Powder
Hazard Statements : H315-H319-H334-H335
MDL No : MFCD00130830
PPE : Dust mask, Eyeshields, Faceshields, Gloves
Safety Symbol : GHS07, GHS08
Source : Clostridium histolyticum
Specific Activity : >100 u/mg
Typical Working Concentration : For typical assay: Dissolve enzyme at a concentration of 1 mg/mL in 0.05 M TES with 0.36 mM calcium chloride, pH 7.5. Dilutions run are 1/10 and 1/20 in the above buffer.
Unit Definition : One unit liberates one µmole of L-leucine equivalents from collagen in 5 hours at 37 °C, pH 7.5.