Collagenase, type I, >125 U/mg
Key Features and Details
From Clostridium histolyticum
Protease which cleaves collagen
For use in the preparation of isolated cell suspensions
SYNONYMS: Clostridiopeptidase A
CAS: #
2593923
EC No: 232-582-9
MDL No: MFCD00130830
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PRODUCT DESCRIPTION
Collagenase, Type I
Application Notes
Collagenase from Clostridium histolyticum has extensive use in biological studies, where it is used to prepare isolated cell suspensions.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Therapeutic uses
SPECIFICATIONS
SKU: 02195109-CF
Base Catalog Number: 195109
Alternate Names: Clostridiopeptidase A
Biochemical Physiological Actions
: Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N',N'-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.The collagenase product is a mixture of enzymes secreted by C. histolyticum, with different products differentiated by the relative ratios of the 10-18 components found in the secreted enzymes. The main components are two collagenases, clostripain, and a neutral protease. The synergistic action of these enzymes degrade collagen and other intracellular materialThe action of both collagenase enzymes and the neutral protease is necessary for effective release of cells from tissue. Various types of collagen are the natural substrates for collagenase.
CAS
: #
2593923
EC No
: 232-582-9
Format
: Lyophilized powder
MDL No
: MFCD00130830
PPE
: Dust mask , Eyeshields, Faceshields, Gloves
Protein or Enzyme Type
: Proteins, Enzymes & Peptides
Safety Symbol
: GHS07, GHS08
Source
: Clostridium histolyticum
Specific Activity
: >125 u/mg
Unit Definition
: One unit will liberate peptides from collagen equivalent in ninhydrin color to 1.0 µmole of L-leucine in 5 hr at pH 7.4 and 37 °C in presence of calcium ions. Contains clostripain, neutral protease and trypsin activities.
