Deoxyribonuclease I, >50,000 U/mg 10 MU
Key Features and Details
SYNONYMS: DNase I;Deoxyribonucleate 5'-oligonucleotido-hydrolase; Deoxyribonuclease A
CAS: # 9003-98-9
EC No: 232-667-0
MDL No: MFCD00130918
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Deoxyribonuclease I Application Notes DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Marker | Cell lysis | Enzymes | Inhibitors | Substrates
Base Catalog Number: 19006210
Alternate Names: DNase I;Deoxyribonucleate 5'-oligonucleotido-hydrolase; Deoxyribonuclease A
CAS : # 9003-98-9
EC No : 232-667-0
Extinction Coefficient : 11.1 at 280 (Lit.)
Format : Powder
MDL No : MFCD00130918
PPE : Eyeshields, Gloves, Respirator filter
Preparation Method : Pancreatic deoxyribonuclease is unusually sensitive to physical denaturation by shaking. Mixing should be done by gentle inversion. Dissolve the standard in 1.0 mL of reagent grade water. Dilute further to a concentration of 20-60 u/mL. All dilutions are made in reagent grade water.
RTECS No : RF0750000
Source : Bovine Pancreas
Specific Activity : >50,000 Dornase u/mg solid
Typical Working Concentration : Minimum inhibitory concentrations (MIC) can range from 0.06 - 1 µg/mL for the most sensitive organisms, and from 4 - 12.5 µg/mL for moderately sensitive organisms.
Unit Definition : Amount of enzyme that causes the fall of 1.0 relative viscosity unit in a solution of highly polymerized DNA in 10 minutes at 30 °C from the intial relative viscosity of 4.0.