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E-64
Key Features and Details
E-64
SYNONYMS: [L-3-trans-Carboxyoxiran-2-carbonyl]-L-leucyl-agmatin; trans-Epoxysuccinyl-L-leucylamido (4-guanidino) butane; N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide; L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatine
CAS: #
66701-25-5
MOLECULAR FORMULA: C15H27N5O5
MOLECULAR WEIGHT: 357.411 g/mol
BEILSTEIN REGISTRY No.: 1405664
MDL No: MFCD00080261
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PRODUCT DESCRIPTION
E-64
Application Notes
E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Inhibitor for thiol protease
SPECIFICATIONS
SKU: 02152846-CF
Base Catalog Number: 152846
Alternate Names: [L-3-trans-Carboxyoxiran-2-carbonyl]-L-leucyl-agmatin; trans-Epoxysuccinyl-L-leucylamido (4-guanidino) butane; N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide; L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatine
Beilstein Registration No
: 1405664
Biochemical Physiological Actions
: E-64 is an irreversible, potent, and highly selective cysteine protease inhibitor. E-64 does not react with the functional thiol group of non-protease enzymes, such as L-lactate dehydrogenase or creatine kinase. E-64 will not inhibit serine proteases (except trypsin) like other cysteine protease inhibitors, leupeptin and antipain. The trans-epoxysuccinyl group (active moiety) of E-64 irreversibly binds to an active thiol group in many cysteine proteases, such as papain, actinidase, and cathepsins B, H, and L to form a thioether linkage. E-64 is a very useful cysteine protease inhibitor for use in in vivo studies because it has a specific inhibition, it is permeable in cells and tissues and has low toxicity.
CAS
: #
66701-25-5
Format
: Powder
MDL No
: MFCD00080261
Molecular Formula
: C15H27N5O5
Molecular Weight
: 357.411 g/mol
Optical Rotation: (c=0.5, 0.1 N HCl): +24.9 ° (Lit.)
PPE
: Eyeshields, Gloves, Respirator filter
RTECS No
: RR0390000
Source
: Synthetic
Typical Working Concentration
: Indirect ELISA: 1:80,000; quantitative precipitin assay: 2.0 mg/mL
