Fluorescamine
Application Notes
Fluorescamine is a reagent for fluorescent assay of amino acids, peptides, proteins and other primary amines. It is also used for fluorometric assays of some proteolytic enzymes. it effectively blocks newly generated amino termini in protein sequence analyses by the Beckman automated sequencer. This product has been used in labeling casein so that it can be used as a substrate for measuring protease activity. Fluorescamine is used in many sensitive detection methods, eg. characterization of poly-L-lysine (pLL)/DNA complexes post-modified with a multivalent hydrophilic polymer, spectrofluorimetric analysis of procaine, detection and quantitation of residual aminopenicillins by HPLC after fluorescamine derivation, determination of lisinopril in human plasma and urine and sulfonamides in honey by HPLC with fluorescence detection.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Fluorometric assay