Guanidine hydrochloride, molecular biology reagent, >99%

Guanidine hydrochloride consists of a networkof guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds,it is a strong chaotropic agent. Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a free flowing powder with solid material dispersed throughout, or a solid. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine HCl appear identical. Application Notes Guanidine hydrochloride can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA.Guanidine hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of protein, it can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Chaotropic agent