Iodoacetamide

Iodoacetamide Application Notes Iodoacetamide is useful in peptide sequencing and as an irreversible enzyme inhibitor. Some enzymes iodoacetamide will inhibit include (but is not limited to): Alcohol dehydrogenase, Alkaline phosphatase (calf intestinal), beta-Amylase, Cathepsin B, Galactose oxidase, Heme oxygenase, L-Lysine decarboxylase. It will irreversibly inhibit the dephosphorylation of both epidermal growth factor receptor and platelet-derived growth factor receptor by alkylation at the active center of one or several protein tyrosine phosphatases in cell lines. Iodoacetamide will also irreversibly inhibit many cysteine proteases. Alkylating reagent for cysteine residues in peptide sequencing. By virtue of reaction with cysteine, it is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Enzymes | Inhibitors | Substrates

Typical Working Concentration : Cell culture substratum at 1-5 µg/cm2 or 0.5-50 µg/mL