Isopropyl-ß-D-thiogalactopyranoside, molecular biology, =99%
Key Features and Details
Isopropyl-?-D-Thiogalactopyranoside
SYNONYMS: IPTG; Isopropyl ß-D-thiogalactoside; l-Propyl-b-D-thiogalactopyranoside
CAS: #
367-93-1
MOLECULAR FORMULA: C9H18O5S
MOLECULAR WEIGHT: 238.298 g/mol
BEILSTEIN REGISTRY No.: 4631
EC No: 206-703-0
MDL No: MFCD00063273
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PRODUCT DESCRIPTION
Isopropyl-?-D-Thiogalactopyranoside
Application Notes
Isopropyl b-D-1-thiogalactopyranoside is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of ß-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the ß-galactosidase coding gene lacZ.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
ß-Galactoside inducer; protein expression
SPECIFICATIONS
SKU: 02194029-CF
Base Catalog Number: 194029
Alternate Names: IPTG; Isopropyl ß-D-thiogalactoside; l-Propyl-b-D-thiogalactopyranoside
Beilstein Registration No
: 4631
CAS
: #
367-93-1
EC No
: 206-703-0
Grade
: Molecular Biology
Hazard Statements
: H319-H351
MDL No
: MFCD00063273
Melting Point
: 121 °C
Molecular Formula
: C9H18O5S
Molecular Weight
: 238.298 g/mol
Optical Rotation: (c=1, water)-26 ° to -33 °
Purity
: =99%
Safety Symbol
: GHS07,GHS08
Sterilization of Solutions
: Solutions can be sterile filtered using a 0.2 mm filter.
Typical Working Concentration
: The final concentration typically used in indicator plates is 0.2 mM.
