Kanamycin monosulfate, USP grade
Key Features and Details
Kanamycin Monosulfate
SYNONYMS: Kanamycin A; Kanamycin sulfate salt
CAS: #
25389-94-0
MOLECULAR FORMULA: C18H36N4O11 • H2SO4
MOLECULAR WEIGHT: 582.575 g/mol
EC No: 246-933-9
MDL No: MFCD00070253
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PRODUCT DESCRIPTION
Kanamycin Monosulfate
Application Notes
Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Antibiotic | Inhibitor of protein synthesis
SPECIFICATIONS
SKU: 02199853-CF
Base Catalog Number: 199853
Alternate Names: Kanamycin A; Kanamycin sulfate salt
Biochemical Physiological Actions
: Kanamycin is an aminoglycoside antibiotic. Aminoglycosides work by binding to the bacterial 30S ribosomal subunit, causing misreading of t-RNA, leaving the bacterium unable to synthesize proteins vital to its growth. Specifically Kanamycin binds to four nucleotides of 16S rRNA and a single amino acid of protein S12. This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. This region interacts with the wobble base in the anticodon of tRNA. This leads to interference with the initiation complex, misreading of mRNA so incorrect amino acids are inserted into the polypeptide leading to nonfunctional or toxic peptides and the breakup of polysomes into nonfunctional monosomes.
CAS
: #
25389-94-0
EC No
: 246-933-9
Grade
: USP
Hazard Statements
: H360
MDL No
: MFCD00070253
Molecular Formula
: C18H36N4O11 • H2SO4
Molecular Weight
: 582.575 g/mol
Potency
: >750 µg/mg
PPE
: Eyeshields, Gloves, Respirator filter
RTECS No
: NZ3225030
Safety Symbol
: GHS08
Source
: Streptomyces kanamycetikus
Typical Working Concentration
: cell culture applications at 100 mg/mL.
