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O-Nitrophenyl-ß-D-galactopyranoside
Key Features and Details
O-Nitrophenyl-?-D-Galactopyranoside
SYNONYMS: o-Nitrophenyl-b-D-galactoside; 2-Nitrophenyl-b-D-galactopyranoside; ONP-b-D-gal; ONPG
CAS: #
369-07-3
MOLECULAR FORMULA: C12H15NO8
BEILSTEIN REGISTRY No.: 92207
EC No: 206-716-1
MDL No: MFCD00063255
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PRODUCT DESCRIPTION
O-Nitrophenyl-?-D-Galactopyranoside
Application Notes
o-Nitrophenyl-ß-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Substrate for ß-galactosidase
SPECIFICATIONS
SKU: 02102473-CF
Base Catalog Number: 102473
Alternate Names: o-Nitrophenyl-b-D-galactoside; 2-Nitrophenyl-b-D-galactopyranoside; ONP-b-D-gal; ONPG
Beilstein Registration No
: 92207
Biochemical Physiological Actions
: ß-galactosidase, breaks down lactose into galactose and glucose. ß-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl ß-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
CAS
: #
369-07-3
EC No
: 206-716-1
Format
: Crystalline
MDL No
: MFCD00063255
Molecular Formula
: C12H15NO8
Optical Rotation: -66 ° ± 7 ° (c=1, H2O)
PPE
: Eyeshields, Gloves, respirator filter
UV Visible Absorbance
: 262 ± 3 nm ? max (H2O)
