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O-Nitrophenyl-ß-D -galactoside, 5 g
Key Features and Details
SYNONYMS: o-Nitrophenyl-ß-D -galactoside; 2-Nitrophenyl -D-galactopyranoside; ONPG; 2-Nitrophenyl ß-D-galactopyranoside
CAS: #
369-07-3
MOLECULAR FORMULA: C
12
H
15
NO
8
MOLECULAR WEIGHT: 301.3
BEILSTEIN REGISTRY No.: 92207
EC No: 206-716-1
MDL No: MFCD00063255
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PRODUCT DESCRIPTION
o-Nitrophenyl ß-D-galactopyranoside is a colorimetric substrate for ß-galactosidase.
Application Notes
o-Nitrophenyl ß-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of ß-galactosidase. It is also used as a cell culture component for molecular genetics.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture component for molecular genetics | Substrate for ß-galactosidase
SPECIFICATIONS
SKU: 0219403005
Base Catalog Number: 19403005
Alternate Names: o-Nitrophenyl-ß-D -galactoside; 2-Nitrophenyl -D-galactopyranoside; ONPG; 2-Nitrophenyl ß-D-galactopyranoside
Beilstein Registration No
: 92207
Biochemical Physiological Actions
: ß-galactosidase, breaks down lactose into galactose and glucose. ß-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl ß-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
CAS
: #
369-07-3
EC No
: 206-716-1
MDL No
: MFCD00063255
Melting Point
: 190 - 202°C
Molecular Formula
: C
12
H
15
NO
8
Molecular Weight
: 301.3
PPE
: Eyeshields, Gloves, respirator filter
UV Visible Absorbance
: UV/VIS: ? max (water): 262 ± 3 nm
