P-Nitro blue tetrazolium chloride, molecular biology reagent
Key Features and Details
P-Nitro Blue Tetrazolium Chloride
SYNONYMS: 2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT
CAS: #
298-83-9
MOLECULAR FORMULA: C40H30N10O6 • 2Cl
MOLECULAR WEIGHT: 817.644 g/mol
BEILSTEIN REGISTRY No.: 4115923
EC No: 206-067-4
MDL No: MFCD00012159
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PRODUCT DESCRIPTION
P-Nitro Blue Tetrazolium Chloride
Application Notes
p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Colorimetric detection of alkaline phosphatase conjugates
SPECIFICATIONS
SKU: 02193999-CF
Base Catalog Number: 193999
Alternate Names: 2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT
Beilstein Registration No
: 4115923
CAS
: #
298-83-9
EC No
: 206-067-4
Extinction Coefficient
: 260 nm: E1% = 740 (in water) (Lit.)
Format
: Powder
Grade
: Molecular Biology
Hazard Statements
: H302
MDL No
: MFCD00012159
Molecular Formula
: C40H30N10O6 • 2Cl
Molecular Weight
: 817.644 g/mol
PPE
: Eyeshields, Gloves, respirator filter
RTECS No
: XF8045000
Safety Symbol
: GHS07
UV Visible Absorbance
: 255 ± 3 nm ( ? max (methanol))
