Papain, technical grade, >1750 USP U/mg
Key Features and Details
Papain
SYNONYMS: Papayotin; Vegetable Pepsin; Caroid; Vermizym; Papainase
CAS: #
9001-73-4
MOLECULAR WEIGHT: 226.236 g/mol
EC No: 232-627-2
MDL No: MFCD00131791
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PRODUCT DESCRIPTION
Papain
Application Notes
Papain is widely used in cell isolation. With some tissues papain has proved less damaging and more effective than other proteases. Of the enzymes used for dissociating turtle retina, papain produced the least trauma. Intact single photoreceptor cells have also been isolated from adult salamander retina with papain. Papain used to obtain high yields of viable, morphologically intact cortical neurons from postnatal rats. Used for dissociation of postnatal rat hippocampus. Papain is used with fetal as well as postnatal brain regions to provide maximal dissociation and viability of neurons. It is also used to produce Fab fragments of antibodies. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
For production of Fab fragments of antibodies | Used for cell dissociation
SPECIFICATIONS
SKU: 02102566-CF
Base Catalog Number: 102566
Alternate Names: Papayotin; Vegetable Pepsin; Caroid; Vermizym; Papainase
Biochemical Physiological Actions
: Papain is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. It is also hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
CAS
: #
9001-73-4
EC No
: 232-627-2
Extinction Coefficient
: 25.0 (Lit.)
Format
: Powder
Grade
: Technical
Hazard Statements
: H315-H319-H334-H335
Isoelectric Point
: pH 9.6 (Lit.)
MDL No
: MFCD00131791
Molecular Weight
: 226.236 g/mol
PPE
: Dust mask, Eyeshields, Faceshields, Gloves
RTECS No
: RU4950000
Safety Symbol
: GHS07, GHS08
Specific Activity
: >1750 USP u/mg