Key Features and Details
SYNONYMS: Polygalacturonase; EC 184.108.40.206; Poly-[1,4-alpha-D-galacturonide] glycanohydrolase; Macerozyme R-10
CAS: # 9032-75-1
EC No: 232-885-6
MDL No: MFCD00131809
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Pectinase Application Notes Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Used in plant protoplast preparation to digest cell wall
Base Catalog Number: 198979
Alternate Names: Polygalacturonase; EC 220.127.116.11; Poly-[1,4-alpha-D-galacturonide] glycanohydrolase; Macerozyme R-10
Biochemical Physiological Actions : Pectinase causes random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
CAS : # 9032-75-1
EC No : 232-885-6
Format : Powder
MDL No : MFCD00131809
PPE : Eyeshields, Gloves, respirator filter
Source : Asperigillus niger
Specific Activity : >1000 u/gm
Typical Working Concentration : A concentration of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase), 24 °C to 37 °C for periods of 1 to 16 hours will yield good results for protoplasting.
Unit Definition : One unit will liberate one µmole of galacturonic acid from polygalacturonicacid per minute at pH 4.0 at 25 °C.