Pepsin, =1:10,000

Key Features and Details

Acid protease used to digest proteins
Commonly used in the preparation of Fab fragments from antibodies
Contains the “cathepsin” component which has milk curdling activity
Broad range of substrate activity and demonstrates an esterase activity

CAS: # 9001-75-6
EC No: 232-629-3
MDL No: pH 1.0

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Pepsin Application Notes Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Pepsin is used as a proteolytic enzyme; used to break down the proteins into their components; i.e.; peptides and aminoacids
SKU: 02102598-CF
Base Catalog Number: 102598
Alternate Names: Pepsin A
Biochemical Physiological Actions : Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the ß chain of insulin.Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.
CAS : # 9001-75-6
EC No : 232-629-3
Extinction Coefficient : E1%280 = 14.7
Format : Powder
Hazard Statements : H315-H319-H334-H335
MDL No : pH 1.0
pKa : 2.5-4.5 (2% aq soln)
PPE : Dust Mask, Eyeshields, Faceshields, Gloves
Protein or Enzyme Type : Proteins, Enzymes & Peptides
RTECS No : SC6132000
Solubility : Freely soluble in water; dissolves readily in 0.01 M HCl (0.5 mg/mL - clear, colorless solution); practically insoluble in alcohol, chloroform or ether.
Source : Porcine
Specific Activity : =1:10,000
Storage : 21

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