Pepsin, >2,000 U/mg protein
Key Features and Details
Acid protease used to digest proteins
Commonly used in the preparation of Fab fragments from antibodies
Contains the “cathepsin” component which has milk curdling activity
Broad range of substrate activity and demonstrates an esterase activity
SYNONYMS: Pepsin A
CAS: #
9001-75-6
EC No: 232-629-3
MDL No: MFCD00081840
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PRODUCT DESCRIPTION
Pepsin
Application Notes
Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Pepsin is used as a proteolytic enzyme; used to break down the proteins into their components; i.e.; peptides and aminoacids
SPECIFICATIONS
SKU: 02195367-CF
Base Catalog Number: 195367
Alternate Names: Pepsin A
Biochemical Physiological Actions
: Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the ß chain of insulin.Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.
CAS
: #
9001-75-6
EC No
: 232-629-3
Extinction Coefficient
: E1%280 = 14.7
Format
: Lyophilized powder
Hazard Statements
: H315-H319-H334-H335
Isoelectric Point
: pH 1.0
MDL No
: MFCD00081840
PPE
: dust mask, Eyeshields, Faceshields, Gloves
Protein or Enzyme Type
: Proteins, Enzymes & Peptides
RTECS No
: SC6132000
Safety Symbol
: GHS07, GHS08
Source
: Porcine Stomach Mucosa
Specific Activity
: >2,000 u/mg protein
Unit Definition
: One unit will produce a delta A280 of 0.001 per minute at pH 2.0 and 37°C, measured as TCA-soluble products using hemoglobin as substrate
