PIPES, cell culture reagent, 1 kg
Key Features and Details
Pipes
SYNONYMS: Piperazine-N,N´-bis[2-ethanesulfonic acid]; Piperazine-N,N; 1,4-Piperazinediethanesulfonic acid; Piperazine-1,4-bis(2-ethanesulfonic acid); Piperzine-N,N'-bis(ethanesulfonic acid)
CAS: #
5625-37-6
MOLECULAR FORMULA: C8H18N2O6S2
MOLECULAR WEIGHT: 302.36 g/mol
BEILSTEIN REGISTRY No.: 817713
EC No: 227-057-6
MDL No: MFCD00006159
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PRODUCT DESCRIPTION
Pipes
Application Notes
PIPES is frequently used as a buffering agent in biochemistry; it is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Zwitterionic Buffer
SPECIFICATIONS
SKU: 0219455291
Base Catalog Number: 19455291
Alternate Names: Piperazine-N,N´-bis[2-ethanesulfonic acid]; Piperazine-N,N; 1,4-Piperazinediethanesulfonic acid; Piperazine-1,4-bis(2-ethanesulfonic acid); Piperzine-N,N'-bis(ethanesulfonic acid)
Beilstein Registration No
: 817713
CAS
: #
5625-37-6
EC No
: 227-057-6
Format
: Powder
Grade
: Cell Culture
MDL No
: MFCD00006159
Molecular Formula
: C8H18N2O6S2
Molecular Weight
: 302.36 g/mol
pKa
: 6.8 at 25 °C; 6.66 at 37 °C(Lit.)
PPE
: Eyeshields, Gloves, respirator filter
Sterilization of Solutions
: it is recommended to filter sterilize using a 0.2 um filter.
UV Visible Absorbance
: OD260 (0.1M in 0.2M NaOH): =0.05; OD280 (0.1M in 0.2M NaOH): =0.05
