PIPES, molecular biology reagent, =99%

Key Features and Details


SYNONYMS: Piperazine-N,N´-bis[2-ethanesulfonic acid]; Piperzine-N,N' bis(ethanesulfonic acid); 1,4-Piperazinediethanesulfonic acid
CAS: # 5625-37-6
MOLECULAR WEIGHT: 302.36 g/mol
EC No: 227-057-6
MDL No: MFCD00006159
SKU 02194838-CF Categories , , Tag

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Pipes Application Notes PIPES is use to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue tissue samples. This will help prevent the loss of lipids. It is also use to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy. PIPES tends to give optimal results. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Buffer | Fluorescence microscopy | Electron microscopy
SKU: 02194838-CF
Base Catalog Number: 194838
Alternate Names: Piperazine-N,N´-bis[2-ethanesulfonic acid]; Piperzine-N,N' bis(ethanesulfonic acid); 1,4-Piperazinediethanesulfonic acid
Beilstein Registration No : 817713
CAS : # 5625-37-6
EC No : 227-057-6
Format : Powder
Grade : Molecular Biology
Hazard Statements : H317
MDL No : MFCD00006159
Molecular Formula : C8H18N2O6S2
Molecular Weight : 302.36 g/mol
pKa : pKa2: 6.8 at 25 °C; 6.66 at 37 °C(Lit.)
PPE : Eyeshields, Gloves, respirator filter
Preparation Method : Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH.
Purity : =99%
Sterilization of Solutions : For sterilization it is recommended to filter sterilize using a 0.2 µm filter.
UV Visible Absorbance : UV/VIS: OD260 (0.1M in 0.2M NaOH) =0.05; UV/VIS: OD280 (0.1M in 0.2M NaOH) =0.05

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