Protease, from Staphylococcus aureus V8 strain, ~500 U/mg
Key Features and Details
Used for selective cleavage of proteins for amino acid sequence determination or peptide mapping
Cleaves peptide bonds on the carboxyl (COOH) terminal side of aspartic and glutamic acid residues
SYNONYMS: Endoproteinase Glu-C
CAS: #
66676-43-5
MDL No: MFCD00132092
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PRODUCT DESCRIPTION
Protease
Application Notes
Protease V8 is used for selective cleavage of proteins for amino acid sequence determination or peptide mapping.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Peptide Mapping
SPECIFICATIONS
SKU: 02151972-CF
Base Catalog Number: 151972
Alternate Names: Endoproteinase Glu-C
CAS
: #
66676-43-5
Extinction Coefficient
: E1%280 = 4.26
Format
: Powder
Hazard Statements
: H315-H319-H334-H335
MDL No
: MFCD00132092
Preparation Method
: The enzyme was isolated, by the method of Drapeau et al., from the culture filtrate of S. aureus V8 strain. The preparation is homogenous on ultracentrifugation and disc electrophoresis.
Safety Symbol
: GHS07, GHS08
Source
: Staphylococcus aureus V8 strain
Specific Activity
: ~500 u/mg
Unit Definition
: One unit will hydrolyze casein to change A280 0.001 per minute, pH 7.8, at 37 °C.