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Protease inhibitor cocktail kit, 1 kit
Key Features and Details
Protease Inhibitor Cocktail Kit
CAS: #
Not applicable
EC No: 200-664-3
MDL No: MFCD00677817
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PRODUCT DESCRIPTION
Protease Inhibitor Cocktail Kit
Application Notes
Protease Inhibitor Cocktail Kit contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. It has been tested on a wide variety of cells and tissue extracts to isolate/maintain intact proteins without degradation.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Protease Inhibitor
SPECIFICATIONS
SKU: 0215883701
Base Catalog Number: 15883701
Biochemical Physiological Actions
: Proteases are enzymes which direct their action upon proteins, cleaving their targets into smaller fragments consisting of polypeptides, peptides and amino acids. There are a myriad of proteases active against practically all proteins. Some proteases are quite specific, while others cleave many different proteins. MP Protease Inhibitor Cocktail covers a broad spectrum of protese inhibitors.
CAS
: #
Not applicable
Components
: 193503-AEBSF(40 mg), 151553- Leupeptin (0.1 mg), 195368- Pepstatin(0.1 mg),195173- EDTA-Na2(500 mg)
EC No
: 200-664-3
Hazard Statements
: Class 8
MDL No
: MFCD00677817
Preparation Method
: Place the contents of all four vials in one volumetric flask or other suitable container. Add water or buffer of choice to 100 mL. More concentrated solutions can be prepared as desired; however, leupeptin and pepstatin A will create more of a suspension at concentrations other than the suggested amount. The prepared solution is stable for 60-90 days when stored at 5 °C. Do not freeze the prepared solution, and avoid repeated freeze-thaw cycles.
Typical Working Concentration
: Typical working concentration for 100 mL of inhibitor cocktail: AEBSF Hydrochloride:50-400 mg/mL; EDTA disodium dihydrate:0.2-25 mg/mL; Leupeptin Hemisulfate: 0.5-1 mg/mL; Pepstatin A: 0.5-1 mg/mL
