Protocatechuate-3,4-Dioxygenase Application Notes Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae.The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into ß-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Used for enzymatic determination of choline esterase
Base Catalog Number: 151975
CAS : # 9029-47-4
Format : Powder
PPE : Eyeshields, Gloves, respirator filter
Source : Pseudomonas sp.
Specific Activity : =3 u/mg solid
Unit Definition : One unit will oxidize one µmole of protocatechuate to 3-carboxy-cis,cis-muconate per minute at pH 7.5 at 37 °C.