Puromycin dihydrochloride, phytopure™

Key Features and Details

Number: 58-58-2

SYNONYMS: Stylomycin ; 6-dimethylamino-9-(3'-deoxy-3'-(p-methoxy-L-phenylalanyamino)- B-D-ribofuranosyl)-purine; 3'-[a-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride
CAS: # 58-58-2
MOLECULAR FORMULA: C22H29N7O5-2HCl
MOLECULAR WEIGHT: 544.40
BEILSTEIN REGISTRY No.: 3853613
EC No: 200-387-8
MDL No: MFCD00012691
SKU 02194539-CF Categories , , Tag

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PRODUCT DESCRIPTION

An aminonucleoside antibiotic that is toxic to both prokaryotic and eukaryotic cells. It causes premature chain termination in protein synthesis and is widely used in cell culture as a selection agent. Application Notes Puromycin can be used in vitro as a selection agent for cells transfected with puromycin N-acetyl transferase gene (pac). It causes premature chain termination in protein synthesis, and is an inhibitor of aminopeptidase and enkephalinase. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Inhibitor of aminopeptidase and enkephalinase.
SPECIFICATIONS
SKU: 02194539-CF
Base Catalog Number: 194539
Alternate Names: Stylomycin ; 6-dimethylamino-9-(3'-deoxy-3'-(p-methoxy-L-phenylalanyamino)- B-D-ribofuranosyl)-purine; 3'-[a-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride
Beilstein Registration No : 3853613
Biochemical Physiological Actions : Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3'-terminal end of the aminoacyl-tRNA.Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.
CAS : # 58-58-2
EC No : 200-387-8
Format : Powder
Grade : Cell Culture
Hazard Statements : H302
MDL No : MFCD00012691
Molecular Formula : C22H29N7O5-2HCl
Molecular Weight : 544.40
PPE : Dust mask , Eyeshields, Gloves
Safety Symbol : GHS07
Solubility : Soluble (NTP, 1992)
Typical Working Concentration : recommended working concentration in eukaryotic cell culture is 1-10 µg/mL
UV Visible Absorbance : ? max (0.1N HCl) 268 ± 2 nm

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