Ribonuclease A

Key Features and Details

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.

SYNONYMS: Ribonuclease pancreatic; Pancreatic Rnase; Alkaline ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3'-pyrimidinooligonucleotidohydrolase
CAS: # 9001-99-4
EC No: 232-646-6
MDL No: MFCD00132181

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PRODUCT DESCRIPTION

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. Application Notes Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Purification of DNA | Analysis of RNA sequences | RNase protection assays | Hydrolyze RNA contained in protein samples
SPECIFICATIONS
SKU: 02193980-CF
Base Catalog Number: 193980
Alternate Names: Ribonuclease pancreatic; Pancreatic Rnase; Alkaline ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3'-pyrimidinooligonucleotidohydrolase
Biochemical Physiological Actions : Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides.
CAS : # 9001-99-4
EC No : 232-646-6
Extinction Coefficient : E1%280 = ~7.3
Format : Lyophilized powder, Salt-free and protease-free
Grade : Molecular Biology
Hazard Statements : H317
Isoelectric Point : 9.45
MDL No : MFCD00132181
pH : Optimum pH: 7.0 - 7.5
PPE : Eyeshields, Gloves, respirator filter
Preparation Method : Chromatographically Purified
RTECS No : RF0760000
Solubility : Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Source : Bovine Pancrease
Specific Activity : =70 Kunitz units/mg
Specificity : The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
Unit Definition : One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C at pH 5.0.

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