ß-Nicotinamide adenine dinucleotide, oxidized form, cell culture reagent, =98 %
Key Features and Details
ß-Nicotinamide Adenine Dinucleotide, Oxidized Form
SYNONYMS: ß-DPN; NAD; Nadide; ß-NAD; Diphosphopyridine nucleotide; DPN; Coenzyme 1; Cozymase; Nadide; Adenosine 5'-(trihydrogen diphosphate) P'->5'-ester with 3-(aminocarbonyl)-1-ß-D-ribofuranosylpyridinium inner salt; 3-carbamoyl-1-ß-D-ribofuranosylpyridinium hydroxide 5'->5'- ester with adenosine 5'-(trihydrogen pyrophosphate) inner salt
CAS: #
53-84-9
MOLECULAR FORMULA: C21H27N7O14P2·×H2O
MOLECULAR WEIGHT: 663.43 g/mol
EC No: 200-184-4
MDL No: MFCD00036253
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PRODUCT DESCRIPTION
ß-Nicotinamide Adenine Dinucleotide, Oxidized Form
Application Notes
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of ß-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, ß-NADH, is fluorescent whereas ß-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Fluorescent measurement of the oxidized or reduced pyridine nucleotides
SPECIFICATIONS
SKU: 02194714-CF
Base Catalog Number: 194714
Alternate Names: ß-DPN; NAD; Nadide; ß-NAD; Diphosphopyridine nucleotide; DPN; Coenzyme 1; Cozymase; Nadide; Adenosine 5'-(trihydrogen diphosphate) P'->5'-ester with 3-(aminocarbonyl)-1-ß-D-ribofuranosylpyridinium inner salt; 3-carbamoyl-1-ß-D-ribofuranosylpyridinium hydroxide 5'->5'- ester with adenosine 5'-(trihydrogen pyrophosphate) inner salt
Biochemical Physiological Actions
: Electron acceptor. ß-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.
CAS
: #
53-84-9
EC No
: 200-184-4
Extinction Coefficient
: EmM = 18.0 (260 nm, pH 7)(Lit.)
Format
: Powder
Grade
: Cell Culture
MDL No
: MFCD00036253
Molecular Formula
: C21H27N7O14P2·×H2O
Molecular Weight
: 663.43 g/mol
Purity
: =98%
RTECS No
: UU3450000
UV Visible Absorbance
: ? max (water) 259 ± 3 nm
