Tris, molecular biology reagent, =99.95%
Key Features and Details
Tris
SYNONYMS: Trometamol; Tromethamine; Tris-[hydroxymethyl]amino- methane; THAM; 2-Amino-2-(hydroxymethyl)- 1,3-propandiol; THAM; Tris base
CAS: #
77-86-1
MOLECULAR FORMULA: C4H11NO3
MOLECULAR WEIGHT: 121.136 g/mol
BEILSTEIN REGISTRY No.: 741883
EC No: 201-064-4
MDL No: MFCD00004679
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PRODUCT DESCRIPTION
Tris
Application Notes
Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). Tris does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is an auxiliary material in pharmaceutical science.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Buffer | Used as a starting material for polymers; oxazolones (with carboxylic acids) and oxazolidines | Used in direct standardization of a strong acid solution
SPECIFICATIONS
SKU: 02194855-CF
Base Catalog Number: 194855
Alternate Names: Trometamol; Tromethamine; Tris-[hydroxymethyl]amino- methane; THAM; 2-Amino-2-(hydroxymethyl)- 1,3-propandiol; THAM; Tris base
Beilstein Registration No
: 741883
Boiling Point
: 219-220 deg C at 10 mm Hg
CAS
: #
77-86-1
EC No
: 201-064-4
Format
: Crystalline powder
Grade
: Molecular Biology
Hazard Statements
: H315-H319-H335
MDL No
: MFCD00004679
Melting Point
: 171-172 deg C
Molecular Formula
: C4H11NO3
Molecular Weight
: 121.136 g/mol
pH
: pH of 0.1 molar aq soln = 10.4
pKa
: 8.1 at 25 °C(Lit.)
PPE
: Dust mask, Eyeshields, Gloves
Purity
: =95%
RTECS No
: TY2900000
Safety Symbol
: GHS07
Solubility
: 79.1 mg/mL in ethylene glycol; 26 mg/mL in methanol; 14.6 mg/mL in anhyd ethanol; 22 mg/mL in 95% ethanol; 14 mg/mL in dimethyl formamide; 20 mg/mL in acetone; 0.5 mg/mL in ethyl acetate; 0.4 mg/mL in olive oil; 0.1 mg/mL in cyclohexane; 0.05 mg/mL in chloroform; less than 0.05 mg/mL in carbon tetrachloride
Sterilization of Solutions
: Tris solutions can be autoclaved (121 °C, 15 psi, 15 minutes) or sterile filtered.
UV Visible Absorbance
: OD 260 nm (5% aq soln) =0.1
Vapor Pressure
: 2.2X10-5 mm Hg at 25 deg C (est)