Trypsin inhibitor, from soybean, =10,000 BAEE unit
Key Features and Details
Trypsin Inhibitor
SYNONYMS: Kunitz Trypsin Inhibitor; Tia1; STI; SBT1
CAS: #
9035-81-8
EC No: 232-906-9
MDL No: MFCD00082103
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PRODUCT DESCRIPTION
Trypsin Inhibitor
Application Notes
After trypsinizing cells, resuspend cells in 1 mL trypsin inhibitor solution (1mg/ml) for every mL of trypsin solution used for dissociation. Centrifuge the cell suspension at 1000 rpm for 5 minutes. A cell pellet should form. Remove as much of the trypsin inhibitor solution as possible and resuspend the pellet in serum-free medium. Culture cells as desired.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture
SPECIFICATIONS
SKU: 02101113-CF
Base Catalog Number: 101113
Alternate Names: Kunitz Trypsin Inhibitor; Tia1; STI; SBT1
Biochemical Physiological Actions
: Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is = 10x108.
CAS
: #
9035-81-8
EC No
: 232-906-9
Extinction Coefficient
: E1% = 9.94 (280 nm, pH 7.6 buffer) (Lit.)
Format
: Powder
Hazard Statements
: H317-H334
Isoelectric Point
: 4.5 (Lit.)
MDL No
: MFCD00082103
PPE
: Eyeshields, Gloves, respirator filter
Safety Symbol
: GHS08
Source
: Soybean
Specific Activity
: =10,000 BAEE units of inhibition/mg material
Unit Definition
: One unit will inhibit one trypsin BAEE unit. One trypsin unit causes an increase of absorbance at 253 nm of 0.001 per minute at 25 °C.
