Urea, molecular biology reagent, =98%

Key Features and Details


SYNONYMS: Carbamide; Isourea; Carbonyldiamine; Ureophil; Urevert; Carbonyl diamide
CAS: # 57-13-6
MOLECULAR WEIGHT: 60.056 g/mol
EC No: 200-315-5
MDL No: MFCD00008022
SKU 02194857-CF Categories , , Tag

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Urea Application Notes Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins
SKU: 02194857-CF
Base Catalog Number: 194857
Alternate Names: Carbamide; Isourea; Carbonyldiamine; Ureophil; Urevert; Carbonyl diamide
Auto Ignition: Not flammable (USCG, 1999)
Beilstein Registration No : 635724
Biochemical Physiological Actions : Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Boiling Point : Decomposes (NTP, 1992)
CAS : # 57-13-6
Conductivity : 68.4 uMhos (Lit.)
Density : 1.34 at 68° F (USCG, 1999)
EC No : 200-315-5
Format : Powder
Grade : Molecular Biology
MDL No : MFCD00008022
Melting Point : 275° F (NTP, 1992)
Molecular Formula : CH4N2O
Molecular Weight : 60.056 g/mol
pH : 7.2 (10% solution)
PPE : Eyeshields, Gloves, Respirator filter
Purity : =98%
RTECS No : YR6250000
Solubility : >9 [ug/mL]
Typical Working Concentration : The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C.
UV Visible Absorbance : OD260 nm (7 M aq soln): =0.2; OD280 (7M aq soln): =0.2
Vapor Pressure : 1.2X10-5 mm Hg @ 25 deg C

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