Urea, reagent grade

Key Features and Details

Urea

SYNONYMS: Carbamide; Carbonyldiamide
CAS: # 57-13-6
MOLECULAR FORMULA: CH4N2O
MOLECULAR WEIGHT: 60.056 g/mol
BEILSTEIN REGISTRY No.: 635724
EC No: 200-315-5
MDL No: MFCD00008022
SKU 02103209-CF Categories , , Tag

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PRODUCT DESCRIPTION

Urea Application Notes Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels.Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses.Urea is used in cell or tissue culture media to increase the osmolality.Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. Key Applications Metabolic Pathways | Chaotropic Agents | Denaturation | Renaturing proteins
SPECIFICATIONS
SKU: 02103209-CF
Base Catalog Number: 103209
Alternate Names: Carbamide; Carbonyldiamide
Auto Ignition: Not flammable (USCG, 1999)
Beilstein Registration No : 635724
Boiling Point : Decomposes (NTP, 1992)
CAS : # 57-13-6
Density : 1.34 at 68° F (USCG, 1999)
EC No : 200-315-5
Format : Prills
Grade : Reagent
MDL No : MFCD00008022
Melting Point : 275° F (NTP, 1992)
Molecular Formula : CH4N2O
Molecular Weight : 60.056 g/mol
pH : 7.2 (10% solution)
PPE : Eyeshields, Gloves, Respirator filter
RTECS No : YR6250000
Solubility : >9 [ug/mL]
Typical Working Concentration : Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C.
UV Visible Absorbance : =0.2 (OD260 nm & OD280 (7M aq soln))
Vapor Pressure : 1.2X10-5 mm Hg @ 25 deg C

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