Reverse transcription of mRNA followed by PCR (RT-PCR) is a common cDNA cloning method; however, it can be difficult to obtain full-length cDNA using this method. Rapid amplification of cDNA ends (RACE) helps overcome this problem by using an oligo(dT) primer for reverse transcription followed by amplification with specifically designed oligonucleotide primers. The 3'-Full RACE Core Set uses a specifically designed Oligo dT Adaptor Primer for efficient synthesis of cDNA from the 3'-end of poly A+ RNA. The Oligo dT Adaptor Primer also encodes three restriction enzyme recognition sites for easy cloning following RT-PCR. Subsequent amplification with this 3' RACE Kit utilizes the Oligo dT Adaptor Primer in conjunction with a specific primer for the gene of interest. Because the 3'-Full RACE Core Set uses AMV Reverse Transcriptase XL to maximize the length of the first-strand cDNA, it is recommended for use with Takara Taq, Takara Ex Taq, or Takara LA Taq polymerases.