A fast way to purify plasmids for transfection—NucleoSnap Plasmid Midi
Endotoxins are copurified during plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Macherey-Nagel has developed a new snap-off column format and a novel buffer chemistry to enable vacuum-processed isolation of transfection-grade plasmid DNA in a midiprep format. See our NucleoSnap Plasmid Midi tech note showing that you can purify 250 μg of plasmid DNA in only 35 minutes.
Endotoxins are copurified during plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Macherey-Nagel has developed a new snap-off column format and a novel buffer chemistry to enable vacuum-processed isolation of transfection-grade plasmid DNA in a midiprep format. See our NucleoSnap Plasmid Midi tech note showing that you can purify 250 μg of plasmid DNA in only 35 minutes.
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